Mantadakis e, pogka v, voulgarikokota a, tsouvala e, emmanouil m, kremastinou j, et al. Within the recognized limitations of retrospective study design, we report the first nosocomial outbreak of hfmd due to echovirus type 11. These pathogen safety data sheets, regulated under workplace hazardous materials information system whmis legislation, are produced for personnel working in the life sciences as quick. Globally, echovirus 30 e30 is one of the most frequently identified. Detection of echovirus 18 in human breast milk journal. Here, we generated echovirus 11 e11 with resistance to chlorine dioxide clo2 by experimental evolution, and we assessed the associated genotypic and phenotypic traits. Nineteen echovirus 11 e11 and 12 e isolates were isolated. In this case, it is possible that enterovirus transmission occurred through the breast milk. Fulltext pdf unique reassortant of influenza ah7n9 virus associated with severe disease emerging in hong kong. Identification of new genotype of echovirus 19 from. Echovirus definition of echovirus by the free dictionary. There are several different serotypes of echovirus known to infect humans.
Echovirus 7 entry into polarized intestinal epithelial. Epidemics of viral meningitis caused by echovirus 6 and 30 in korea. Fatal infections with echovirus types 6 and 11 in early infancy. Bergelsona,b divisionofinfectiousdiseases,childrenshospitalofphiladelphia,philadelphia,pennsylvania,usa,a anddepartmentofpediatrics,universityofpennsylvania. A report of a springsummer outbreak of echovirus 11 infection. Ev11 viral capsid protein 1 vp1 sequences segregate into six or more genogroups 5, 6. Echovirus 11 infections of newborns with mortality during the 1979 enterovirus season in milwaukee, wis. The first group comprised 12 babies who died aged between. Pdf an outbreak of echovirus meningitis in children.
The echo part of the name is an acronym for enteric cytopathic human orphan viruses. Reaction pattern of immunoglobulin m and g antibodies to. We describe an outbreak of aseptic meningitis that occurred in southern brazil in 2003 with echovirus e virus as the etiologic agent. We describe an outbreak of aseptic meningitis that. Echovirus serotype 11 echo 11 was implicated in three neonatal deaths during an enterovirus outbreak from july through october 1979 in milwaukee. Echovirus 11 definition of echovirus 11 by medical. Echovirus 11 synonyms, echovirus 11 pronunciation, echovirus 11 translation, english dictionary definition of echovirus 11. At the time of the isolation of the viruses the diseases they caused were not known, hence the term orphan, but it is now known that they cause many different types. Enteroviruses are known to cause childhood paralysis. Acute transverse myelitis caused by echovirus 11 in a pediatric. Dec 02, 2015 a large finnish echovirus 30 outbreak was preceded by silent circulation of the same genotype.
Echovirus 7 entry into polarized intestinal epithelial cells. Any member of the picornaviridae family, genus enterovirus. The virus particle lacks an envelope, is spheroidal, measures from 20 to 30 nanometres nm. It can be helpful just to confirm the diagnosis, but. An orphan virus is a virus detected under laboratory conditions but not associated with any known disease. Coxsackie viruses cause sore throat or fever with chest or abdominal pains. A novel open and infectious form of echovirus 1 journal of virology.
Echovirus 11 definition of echovirus 11 by the free. This study describes some of the clinical, virologic and epidemiologic characteristics of the outbreak. Sixtysix enteroviruses and 7 adenoviruses were recovered from 73 persons in a survey hospital population. Vesicular herpetiform eruptions have been linked to coxsackievirus a9 and echovirus 11. American journal of epidemiology, volume 114, issue 3, september 1981. Fatal echovirus type 11 infections jama pediatrics. In 1981 we reported 1 successful treatment of echovirus 11 meningoencephalitis and myositisfasciitis in a patient with xlinked agammaglobulinemia, using a modified. Author summary we describe evolution of echovirus 11 genome in. Differences in viral disinfection mechanisms as revealed. Differences in viral disinfection mechanisms as revealed by. Epidemics of viral meningitis caused by echovirus 6 and 30 in korea in 2008. Detection of echovirus 18 in human breast milk journal of.
In the united states, arup laboratories and cambridge biomedical offer a serum microneutralization assay that is designed to measure the concentration of serum antibodies to five serotypes of the virus. Human echovirus 11 is a member of the human enterovirus b species and is one of the most commonly isolated enteroviruses 3, 4. Echovirus 11 was treated with ozone, free chlorine fc, uv light at 254 nm uv 254, or heat, and then the reductions in genome functionality and. This is the first report of widespread circulation of echovirus and of outbreaks associated with this enterovirus in the united states. Twentyeight persons yielded echovirus type 11, 22 echovirus type 6, and 16 either echovirus type 9 or coxeackieviruses group b, types 2 and 3. Echovirus 30 and coxsackievirus a9 infection among young. The deaths followed congenital infections acquired in the community during late pregnancy. Echovirus 30, jiangsu province, china volume 11, number 4.
Here, we identify the neonatal fc receptor fcrn as a panechovirus receptor. Echovirus 1, another member of the picornavirus family and a cause of febrile illness and meningitis, has been shown to use a human cell integrin. Fatal echovirus 11 infections in outbreak in specialcare baby unit. Ozone reduced genome functionality proportionally to infectivity, indicating that genome damage is the main mechanism of virus inactivation. In korea, echovirus type 30 e30 and e6 have been associated with outbreaks. Ratio of silent to missense mutations in protein coding regions suggests that chronic virus was under lower selective pressure than circulating viruses, except for a. An echo enteric cytopathic human orphan virus is a type of rna virus that belongs to the. Multiple genotypes of echovirus 11 circulated in mainland. However, it has been found that this is not true of echovirus. We found that infection of e5, e11, and e30 was significantly reduced in two clones of u2os. Echovirus 30, jiangsu province, china volume 11, number. Ozone reduced genome functionality proportionally to infectivity, indicating that genome damage is the main mechanism of virus inactiva. Identification of new genotype of echovirus 19 from children. Survival in groundwater of echovirus types 6,11 and 24.
The dense particle was permeable to sybr green ii, it still contained most of its vp4, and it was. This is the first meningitis outbreak due to e reported in the country. Increased echovirus activity also was reported in europe during 2000 when echovirus was associated for the first time with outbreaks of aseptic meningitis in england, wales, and germany 4,5. Echovirus 11 definition of echovirus 11 by medical dictionary. Mar 02, 2020 echovirus infections cant be directly prevented, and theres no specific vaccine available for the echovirus. The temporal dynamics and genetic diversity among strains of the echovirus 30 serotype have been reported in previous studies 1,9,27, but no echovirus isolates from china were included. Echovirus may also produce a rash that spreads from the face down to the neck, upper extremities, and chest. Transmission networks and population turnover of echovirus 30. All were children, and could be divided into two groups according to age at death and clinical. A large finnish echovirus 30 outbreak was preceded by silent circulation of the same genotype.
We detected enteroviral rna and cultured infectious virus from a series of banked breast milk samples from the mother of an infant with neonatal sepsis. These pathogen safety data sheets, regulated under workplace hazardous materials information system whmis legislation, are produced for personnel working in the life sciences as quick safety reference material relating to infectious microorganisms. Jun 14, 2019 vesicular herpetiform eruptions have been linked to coxsackievirus a9 and echovirus 11. Of the 1672 nonpolio enterovirus isolates, echovirus 30 was the predominant serotype and accounted for 27. Minor, national institute for biological standards and. An echovirus measures 2430 nanometres nm and has a naked protein capsid, which makes up 75% of the virus particle. When first discovered, it was labelled as a coxsackie a virus, a23. Two infants with fatal echovirus type 11 infections are described. Renal medullary haemorrhage and echovirus infection. Respiratory tract infection among the echoviruses, serotype 11 is the most firmly established cause of respiratory disease, although serotypes 4, 8, 9, 20, 22, and 25 may also be causal agents. Yes, just a test that looks at levels of long term antibodies against coxsackie b1 through b6 and echovirus 6, 7, 9, 11, and 30. Echovirus 11, the most commonly implicated serotype, is acquired by vertical transmission from mothers in utero or at delivery, or postnatally by nosocomial spread. Bingo positive enterovirus what next health rising. Echoviruses constitute one of the major groups of the genus enterovirus and are associated with illnesses including aseptic meningitis, nonspecific.
Waterborne viruses can exhibit resistance to common water disinfectants, yet the mechanisms that allow them to tolerate disinfection are poorly understood. An echovirus previously written echo, or enteric cytopathic human orphan is a type of enterovirus. The present study is the first to show that a distinct lineage of echovirus 30 caused meningitis in china. Human echovirus 11 frequently causes aseptic meningitis and the clinical syndrome of hand, foot, and mouth disease. The host cellular protein recognized by this integrin is collagen, an. All were children, and could be divided into two groups according to age at death and clinical presentation. Echovirus article about echovirus by the free dictionary. Evolution of echovirus 11 in a chronically infected. The purpose of this study was to examine the genetic diversity and to determine the association of nonpolio enteroviruses npevs with acute. No subsequent cases were identified in the 4 months. Laboratory diagnosis is made with acute and convalescent titers of serum antibodies to echovirus. Disseminated intravascular coagulation developed in both patients, and at postmortem examination, diffuse hemorrhagic necrosis of multiple organ systems was evident, most strikingly in the liver.
Evolution of echovirus 11 in a chronically infected immunodeficient. Echovirus 11 infections of newborns with mortality during. Author summary we describe evolution of echovirus 11 genome in chronically infected immunodeficient patient over a period of several years and compare it with the evolution of. Among the enteroviruses are polioviruses, echoviruses enteric, cytopathogenic, human, orphan, and coxsackie viruses. The neonatal fc receptor is a panechovirus receptor pnas. Echovirus belongs to the genus enterovirus of the family picornavirus. Echovirus 11 was treated with ozone, free chlorine fc, uv light at 254 nm uv 254, or heat, and then the reductions in genome functionality and infectivity were compared. Echovirus, like the other enteroviruses coxsackievirus a and b, typically causes a mild, nonspecific illness with a low fever. Coxsackie virus b5 and echovirus 11 were the predominant serotypes during the study period. Echovirus 11 e 11 is one of the most frequently isolated enteroviruses causing meningitis and other diseases such as hand, foot, and mouth disease hfmd and acute flaccid paralysis afp. Several of the 28 currently recognized serotypes of echovirus are found in association with these infections 3. It was later discovered that a23 was an echovirus antigenically identical to the alreadyknown echovirus 9.
Disseminated intravascular coagulation developed in both patients, and at postmortem examination, diffuse. Echovirus 11 e11 is one of the most frequently isolated enteroviruses causing meningitis and other diseases such as hand, foot, and mouth. Infection occurs in young infants especially in summer and autumn 11. Echovirus may also produce a rash that spreads from the. Respiratory tract infection among the echoviruses, serotype 11 is the most firmly. Echovirus 1 e1, a member of the enterovirus b group, shares the structural. Echovirus, a singlestranded rna enterovirus, belonging to the picornaviridae family, can cause significant neonatal morbidity and mortality. In 1981 we reported 1 successful treatment of echovirus 11 meningoencephalitis and myositisfasciitis in a patient with xlinked agammaglobulinemia, using a modified intravenous. A total of 1,681 cases were involved in this outbreak, and 99% of.
Aug 01, 2006 several of the 28 currently recognized serotypes of echovirus are found in association with these infections 3. A number of enteroviruses, including echoviruses ev and group b. The spread of an echovirus infection can be especially hard to control because. Enteroviruses invade the host by crossing the intestinal mucosa, which is lined by polarized epithelium. Many of the more than 30 serotypes live in the human intestine without causing any symptoms of disease. Next, we utilized crisprcas9mediated gene editing to knock out fcrn expression. Echovirus infections cant be directly prevented, and theres no specific vaccine available for the echovirus. Echovirus 11 infections of newborns with mortality during the. Data from the annual reports of the central virology laboratories, public health service of the israeli ministry of health, tel hashomer israel, indicate that ev11 is endemic in israel with at least one ev11positive case being reported on 17 of the 27 years between 1986 and 2012. Echovirus 11 e11 is one of the most frequently isolated enteroviruses causing meningitis and other diseases such as hand, foot, and mouth disease hfmd and acute. Multiple genotypes of echovirus 11 circulated in mainland china. Twentyfour fatal cases of echo 11 infection in the eleven years 196878 are presented. Whereas immunoglobulin g antibodies were directed most exclusively to the vp 1 protein, the immunoglobulin m antibodies were directed against vp 1, vp 2, and vp 3.
Fatal echovirus type 11 infections jama pediatrics jama. Resistance of echovirus 11 to clo2 is associated with. Echovirus 11 article about echovirus 11 by the free dictionary. No subsequent cases were identified in the 4 months after the nursery unit reopened. Echovirus 30 outbreak associated with a high meningitis attack rate in thrace, greece.
Echovirus 11 was treated with ozone, free chlorine fc, uv light at 254 nm uv254, or heat, and then the reductions in genome functionality and infectivity were compared. Echovirus 11 article about echovirus 11 by the free. The spread of an echovirus infection can be especially hard to. Echovirus definition of echovirus by medical dictionary. Science by american association for the advancement of science 1883. Survival in groundwater of echovirus types 6, 11 and 24. Echovirus 11 definition of echovirus 11 by the free dictionary. An outbreak of aseptic meningitis occurred in the northern area of jiangsu province in china from january to july in 2003. The immunoglobulin m and g specific immune response of humans to echovirus 11 proteins during an echovirus 11 outbreak was studied by the immunoblot technique. Jul 22, 2019 echovirus 11 e 11 is one of the most frequently isolated enteroviruses causing meningitis and other diseases such as hand, foot, and mouth disease hfmd and acute flaccid paralysis afp. Echovirus 7 entry into polarized intestinal epithelial cells requires clathrin and rab7 chonsaeng kima and jeffrey m. A number of enteroviruses, including echoviruses ev and group b coxsackieviruses cvb, initiate infection by attaching to decayaccelerating factor daf, a molecule that is highly expressed on the apical surface of polarized epithelial cells. Author summary we describe evolution of echovirus 11 genome in chronically infected immunodeficient patient over a period of several years and compare it with the evolution of circulating echoviruses from which it originated. Two of the three echo 11 and one coxsackie b4 deaths of infants occurred after cesarean section deliveries.